FastQCFastQC Report
Mon 27 Feb 2023
SRR3127598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399988
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286261.1927559637798189No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC28580.1190839287529771TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27590.11495890812787397TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27470.11445890562786147TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88700.084.929381
ATAGGGC16900.074.4455643
ATAGCGG5350.074.01681
TAGGGCA17700.073.4767764
TAGCGGG11100.072.167262
GAATAGG14400.072.0219651
AGAGGGC41950.070.428733
CGTAGGG7600.070.061452
AGTAAGG17850.069.722111
CGAAGGG23150.069.4092942
AGTAGGG41050.069.2178962
ATAGGGA21850.069.0101243
TACGGGT1300.068.869123
GGATAGG15950.068.8652341
AAGGGTA17350.068.7124254
ATAAGGG37400.068.287612
AATAGGG37100.068.0777362
AGGGATG37300.068.034695
AAGGGAT45050.066.9421164
AGGGTAC12150.066.913745
Produced by FastQC (version 0.11.5)