FastQCFastQC Report
Mon 27 Feb 2023
SRR3127597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150788
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125741.0926426066312822No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG38160.33159886964410473No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG34100.29631869640628855No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC34060.2959711084926155Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17200.14946280287941827No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT12270.10662259251921291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36300.083.807151
TAGTAGG3950.078.565211
AGGGTAC3950.078.524255
GAATAGG6200.075.080451
ATAGGGC6400.074.9024353
CGTAGGG3000.073.632782
TAGACGG1150.073.596661
TATAGGG8200.072.7921452
CGAAGGG9600.070.989062
AAGGGTA6750.070.319294
AGTAGGG18050.070.3041842
GGATAGG4850.069.803011
AATAGGG15450.069.358712
AGGGCAT10250.068.7745
ATAGGGA8550.068.160283
AGGGTAA4500.067.8824845
TAGAGGG17700.067.7113652
TAGGGCA6000.067.360314
AGGGTAT4900.067.136535
TAAGAGG9350.066.8841861
Produced by FastQC (version 0.11.5)