FastQCFastQC Report
Mon 27 Feb 2023
SRR3127595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066164
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123151.1550755793667766No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG32970.30923947910452804No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG30430.28541575217321163No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC27310.2561519616119096Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16440.15419766564993753No Hit
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT11060.10373638577179495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37200.086.14671
TAGTAGG3950.076.358171
AGTAGGG18400.075.788552
CGAAGGG9400.075.6795962
CGTAGGG3500.075.378882
TATAGGG7500.074.750722
GAATAGG4300.073.430921
ATAGGGC6100.073.3708343
AGTAAGG7550.073.031731
AGGGTAC5100.071.874255
TGAAGGG19700.071.026412
AAGGGTA8650.070.803864
TAGAGGG15750.070.592922
GCGATAT409.757241E-870.492058
GTAGGGA9150.070.02413
AAGAGGG32150.068.872612
TAAGGGA8750.068.379423
AATAGGG13250.068.267662
ATAGAGG7450.067.68621
TAGGGAC4250.067.619294
Produced by FastQC (version 0.11.5)