FastQCFastQC Report
Mon 27 Feb 2023
SRR3127594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829637
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175190.9575123371466581No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG28180.1540196224715613No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG27250.14893664699609813No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25370.13866138474462422No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC25240.13795086129106485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52500.086.086771
TAGTCGG353.8489816E-880.622371
TAGGGCA13850.079.064524
GAATAGG10400.078.2321241
CGTAGGG5050.077.245662
AGTACGG2900.072.977141
GGTAAGG9900.071.257141
AGGGATG25950.070.996365
AGTAGGG30000.070.654972
ATAGGGC14850.069.6260453
ATAAGGG24200.069.527252
TGTAGGG14450.069.27862
TAGCGGG9100.069.207122
AGGGTAC10050.068.2769555
GTAGGGA13450.068.137763
ATAGAGG14100.067.709451
TAGTAGG8700.067.571431
TAAGGGA15950.067.476153
GATAGGG25850.067.27112
GTAGGGC9050.066.991033
Produced by FastQC (version 0.11.5)