FastQCFastQC Report
Mon 27 Feb 2023
SRR3127593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201476
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236191.0728711101097628No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31240.14190479478313642No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG28620.13000368843448667No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG27750.12605179434161445No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC25240.11465035276332787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67150.086.252651
CGTAGGG5900.082.0452
TAGGGCA17550.077.390494
GAATAGG14550.075.6681751
TAGTCGG605.456968E-1270.575121
TAGGGTA8200.069.9218444
ATAGGGC17550.069.892453
AGGGCAT24200.069.329775
AGTAGGG36500.069.143012
ATAGAGG16000.069.1048051
TAGGGTC5650.069.039334
AGGGATG34250.068.745465
TGTAGGG16300.068.620872
AAGGGCA42300.067.772944
ATAAGGG31400.067.501562
AGGGCAA29750.067.2960365
TAGAGGG41350.067.170542
AGAGGGC35300.066.700633
AGGGTAC10800.066.578595
GGTAAGG15050.066.276531
Produced by FastQC (version 0.11.5)