FastQCFastQC Report
Mon 27 Feb 2023
SRR3127592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676300
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173561.035375529439838No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG25090.14967487919823422No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24170.14418660144365567No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG23550.1404879794786136No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC21710.12951142396945653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47200.087.1478961
CGTAGGG4650.078.9890062
TAGGGCA11450.076.4948964
TAGACGG1500.075.388431
AGGGATG24950.073.836615
GAATAGG10050.072.669191
GAGGGAT16750.071.969794
AGTAGGG28050.070.676382
AGGGCAT17050.070.010895
AGACGGG9750.068.0988162
ATAGGGC11900.068.062283
AGTAAGG11700.067.656281
AAGGGAT29800.067.632044
ATAAGGG22350.067.421362
AAGGGCA31500.067.1213764
TAGCGGG8450.066.315542
AGGGCAA22050.066.28395
AAGAGGG62000.066.0013962
GTAGGGT6650.065.854633
AGAGGGC25400.065.81423
Produced by FastQC (version 0.11.5)