FastQCFastQC Report
Mon 27 Feb 2023
SRR3127591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716808
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316291.164197101893104No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39800.1464954461264837No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG29390.10817842114716977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98850.084.320651
CGTAGGG7450.076.329462
ATAGCGG5600.073.076571
TAGGGCA18850.070.555394
ATAGGGC16950.069.8705143
GAATAGG16400.069.6962361
AGGGATG45350.069.53455
AGTAGGG45350.069.4321442
TAGCGGG16000.069.319452
AGAGGGC42200.068.601143
ATAGGGA21650.068.377893
AAGAGGG98650.067.171512
AGGGCAT28900.066.834245
TAGGGAT17400.066.711824
TAGAGGG46850.066.10572
AAGGGAT46900.066.034014
ATAAGGG43250.065.9577262
ACGGGTA3650.065.6647644
TATAGGG22100.065.284362
TCGTAGG2100.064.956941
Produced by FastQC (version 0.11.5)