FastQCFastQC Report
Mon 27 Feb 2023
SRR3127590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244407
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411931.2696619135638654No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51250.157964151846547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129900.084.3791661
CGTAGGG8900.076.040642
TAGGGCA22200.075.3662644
ATAGCGG7550.072.899971
GAATAGG19200.072.523541
AGTAGGG52200.071.036222
AGGGATG57450.070.189245
AGAGGGC54650.069.830613
ATAGGGC22300.068.916453
AAGGGAT57500.068.249374
TAGAGGG62800.067.951582
AAGAGGG122200.067.342352
AGGGCAT33900.067.099455
TAGCGGG19050.066.610412
AAGGGTA26050.066.5730744
GAGGGAT40950.066.221934
ATAGAGG25400.066.118491
CGAAGGG33750.065.726652
AGGGTAC15150.065.455055
AGAGGGA74950.065.339543
Produced by FastQC (version 0.11.5)