FastQCFastQC Report
Mon 27 Feb 2023
SRR3127589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474577
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308481.2465968931255726No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35810.14471160121507634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96000.085.061131
CGTAGGG5800.079.587252
TAGGGCA16200.073.852314
AGGGATG42000.071.052135
TAGAGGG43800.070.331362
AGAGGGC42550.068.965823
TAGCGGG13950.068.881312
GAATAGG15300.068.6779251
AGTAAGG15850.068.07851
ATAGGGA21500.067.915463
CGAAGGG26750.067.440442
AGTAGGG39550.067.408612
TAGGGTA9200.066.5581054
AGATAGG15200.066.339771
ATGCGGG11900.066.102052
GAGGGAT30900.066.00474
AAGGGAT44000.065.943724
AAGGGCA42700.065.524544
AAGAGGG92850.065.340032
ATAGAGG17600.065.0574951
Produced by FastQC (version 0.11.5)