FastQCFastQC Report
Mon 27 Feb 2023
SRR3127588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724422
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171210.9928544173062046No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG38310.22216139668828164Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG35920.20830168021516776Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC34580.20053096051894492Illumina PCR Primer Index 2 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23140.13418989087357966No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17990.10432481144406647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50250.085.745411
CGTAGGG3650.078.543532
GGTAAGG9500.075.7563861
TAGGGCA10350.074.9233254
TATAGGG14300.073.618252
GAATAGG9550.073.389571
ATAGGGC12600.072.734013
AGGGTAC7900.071.98335
AGTAGGG29850.071.637522
GTAGGGT7350.069.696773
ATAGGGA14650.069.293043
TAGAGGG31950.069.13542
AGTAAGG11150.068.764481
AGTACGG1650.068.419321
GTATAGG8100.067.944181
ATAGCGG3050.067.8585051
TAGTAGG8950.067.7981951
AAGAGGG58550.067.58632
ATAGAGG13100.067.505321
AAGGGTA10750.067.326454
Produced by FastQC (version 0.11.5)