FastQCFastQC Report
Mon 27 Feb 2023
SRR3127587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045511
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226771.1086227353458378No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG36890.18034613355782492Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC35580.17394186587116864Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG34910.1706664007184513Illumina PCR Primer Index 2 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27910.13644512300349398No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22360.10931253852949213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64800.084.665441
CGTAGGG5200.077.728182
TAGGGCA12850.076.8067864
GAATAGG12150.075.903611
AGTAGGG35550.073.505292
TAGGGCG4150.072.4795154
ATAGGGC15300.071.879963
TATAGGG18200.071.530592
AAGGGTA15200.071.4252554
ATAGAGG13700.069.7201161
AGGGCAT23600.069.701075
AAGAGGG74400.068.91842
AGGGTAC10200.068.654585
AGTAAGG12100.068.439991
AGAGGGC28550.067.657993
GGATAGG12600.067.591281
TAAGAGG15600.067.562551
GTAGGGT8650.067.3734743
GGTAAGG12650.066.952161
AGGGATG26350.066.8858265
Produced by FastQC (version 0.11.5)