FastQCFastQC Report
Mon 27 Feb 2023
SRR3127586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614734
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167601.0379418529615405No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG33320.20634977649569525Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG31840.1971841801807604Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC29430.18225912131657598Illumina PCR Primer Index 2 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21220.13141483365061984No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16920.1047850605734443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50400.086.029011
GAATAGG9900.074.739831
AGTAGGG30250.074.297262
GTACGGG3850.073.429442
ATAGGGC10050.073.137193
TAGGGCA10650.072.5561144
TAGGGCG2750.071.960854
TATAGGG12450.071.527362
AGTACGG1400.070.6934051
AGGGATG21950.070.010295
AGTAAGG9450.069.820651
ATAGCGG2850.069.453161
GGTAAGG8900.069.369561
CGTAGGG4700.069.1721042
TAGCGGG6850.068.784282
ATAGGGA13900.068.133553
AAGAGGG55550.067.85562
ATAGAGG9400.067.18381
AAGGGTA11100.067.0677644
AGGGCAT14450.066.9958655
Produced by FastQC (version 0.11.5)