FastQCFastQC Report
Mon 27 Feb 2023
SRR3127585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743940
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82441.1081538833776918No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC32590.43807296287335TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG32310.43430921848536175No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG32000.430142215770089No Hit
AGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT9050.1216495953974783No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8930.12003656208834045No Hit
GGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT8430.11331558996693282No Hit
GGAGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT8200.11022394279108531No Hit
ACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG8110.10901416780923193No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT7690.10336855122724951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1800.088.772322
CGTTTTT27100.084.1771241
TAGTAGG4000.081.135461
AGGGAAT9100.080.0554055
ATAGCGG1250.079.018881
ATAGGGC2400.076.37033
GGTAAGG3400.076.086111
CTATAGG1300.075.97971
GGATACG507.094059E-1175.195378
TAAGGGA4950.075.0054863
GTACGGG1100.072.63192
GAATAGG3850.072.079681
ATGTAGG2350.072.053691
ACTAGGG2750.071.7774052
AGGGCAT5650.071.540235
GTAGGGT2500.071.435613
GTCGATG605.456968E-1270.495677
AGTAAGG5000.069.611881
ATAAGGG10150.069.453862
TAGCGGG3200.069.0272
Produced by FastQC (version 0.11.5)