FastQCFastQC Report
Mon 27 Feb 2023
SRR3127584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871512
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111201.2759434178760591No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG33490.3842746858333563No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG31770.36453887037699995No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC31710.36385041169828986TruSeq Adapter, Index 15 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12750.14629746922589706No Hit
AGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT9960.11428414066587723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37150.085.9825061
CGTAGGG2250.081.4530942
GGTAAGG4650.075.8765261
GTAGGGT1950.074.70553
TAGTAGG3850.074.536381
AGGGAAT12300.073.735685
AGTAAGG6000.073.701411
GAATAGG4050.073.17871
TAAGGGA6900.072.1908653
GTAGAGG6850.072.11041
AGGGATT9450.070.612575
ATAGCGG800.070.565171
AGGGTAC2200.070.488265
TATAGGG6750.069.618032
TAGAGGG15500.068.517612
ATAGGGT3650.068.235213
ATAGGGC4100.067.622883
AAGAGGG24400.067.5993962
GTAAGGG14050.067.227232
GTAAAGG5950.067.204931
Produced by FastQC (version 0.11.5)