FastQCFastQC Report
Mon 27 Feb 2023
SRR3127583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681334
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81031.189284550602201No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG29370.43106611441671777No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG28840.4232872570574784No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC28010.4111052728911224TruSeq Adapter, Index 15 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9430.13840495263703265No Hit
AGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT8530.12519557221568278No Hit
GGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT7140.10479441800937572No Hit
GGAGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT7090.10406056354152296No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT6970.10229931281867631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT26450.085.884171
CGTAGGG1650.079.92422
TAAGGGA5050.077.4089053
CGGGTAT507.094059E-1175.191195
AGTAAGG4300.074.53021
GGTAAGG4250.074.2980961
AGGGAAT9550.073.813355
TAGTAGG3300.072.836331
TATAGGG3500.072.665782
AGGGCAT6000.071.274985
TACGAGG1200.070.6940841
GTTAGGG3200.070.6472852
TAGCGGT200.002163605570.491749
GATAGTC200.002163605570.491748
ACGGGAT950.069.407854
GGGCGAT2850.069.255046
GAGGGAT7600.068.788144
ACTAGGG2950.068.6515962
CGAGGGA4050.068.6121753
AATAGGG8450.068.557132
Produced by FastQC (version 0.11.5)