FastQCFastQC Report
Mon 27 Feb 2023
SRR3127582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585880
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253380.9798598542855816No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37070.143355453462651No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG29590.11442913050876298TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG27330.10568935913499466TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC26160.10116478722910575TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74050.084.919861
AGGGATG43150.076.128355
GTACGGG6200.074.285022
CGTAGGG6150.074.124792
TAGCGGG12400.071.252982
TAGGGCA17250.071.105224
GAATAGG13950.070.802411
AAGGGTA21100.070.380884
ATAGGGC17750.070.162653
AGTAGGG47150.069.772322
CGAAGGG25400.069.754862
ATAGCGG5800.069.738621
GGTAAGG15400.069.63331
ATAGGGA23350.069.0344243
AGGGCAT25950.068.4548955
TAGAGGG46650.068.001572
GTAGGGC13050.067.7026753
GTAGGGA21550.066.9498443
AGGGTAC14650.066.723035
GAGGGAT35000.066.464114
Produced by FastQC (version 0.11.5)