FastQCFastQC Report
Mon 27 Feb 2023
SRR3127580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399303
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251261.047220797039807No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31290.13041287407217847No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG25760.10736451377754289TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG24930.10390517579480375TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC24210.10090430429170472TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74050.084.812171
TAGGGCA15950.077.0623864
CGAAGGG23800.073.608612
ATAGCGG5200.073.389651
AAGGGTA17900.071.561354
AGGGATG41100.071.007865
ATAGGGC18450.070.959583
CGTAGGG7450.070.798552
AGTAGGG44400.070.110192
GAATAGG13100.069.7723851
TAGCGGG13000.069.553752
TAGAGGG41800.068.950562
AGGGAAT26750.068.692545
AGGGCAT21000.068.479225
GTACGGG5150.067.668592
TAGGGTC7250.067.555084
GATAGGG37950.067.010772
AAGAGGG92700.066.9065552
AGATAGG15000.066.902371
AGTAAGG16550.066.899521
Produced by FastQC (version 0.11.5)