FastQCFastQC Report
Mon 27 Feb 2023
SRR3127579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658400
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126190.7609141341051615No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG43210.260552339604438No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC39110.2358297153883261Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG36590.22063434635793536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50200.086.2666851
CGTAGGG5250.084.1512
AGGGATG32550.077.2467965
CGAAGGG14300.076.579162
GGGCGAT5350.075.5433356
AGGGCAT20600.074.3751755
CGAGGGA10650.074.139643
CGGGATT5950.072.6688845
AAGGGCA32400.072.527494
AAGGGAT40900.072.507644
GCGGGAT10000.072.376634
ATAGGGC9850.072.049583
AGAGGGC25250.072.034463
TAGGGCA11750.071.596674
GAGGGAT31400.071.2450944
AGGGCAA23050.070.955495
AGTAGGG26750.070.454922
AGGGATT26550.070.275455
AGAGGGA49100.070.2595443
ATAGGGA19500.070.1373143
Produced by FastQC (version 0.11.5)