FastQCFastQC Report
Mon 27 Feb 2023
SRR3127578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009819
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176510.8782382891195675No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG41030.20414773668673644No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC39390.19598779790617962Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG38550.1918083170673578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66650.085.754341
AGTACGG1850.081.3688661
ACGGGTA2450.080.570134
ATAGCGG3900.077.1961061
AGGGATG41850.076.477145
GGGCGAT6300.073.854116
CGAAGGG17100.073.659632
CGTAGGG7050.073.332152
AGAGGGA61200.072.7259453
AAGGGAT50100.072.703424
AGGGCAT24600.072.02555
TAGGGCA15100.071.89954
GCGGGAT13350.071.819064
TAGCGGG10600.071.3856352
AAGGGCA38100.070.807254
AGAGGGC30700.070.72853
ATAGGGA25000.070.686863
CGAGGGA15050.070.5769353
TAGACGG3000.070.562061
AAGAGGG69650.070.3807752
Produced by FastQC (version 0.11.5)