FastQCFastQC Report
Mon 27 Feb 2023
SRR3127577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543088
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128160.8305423929160229No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG37410.24243594662132034No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC33820.21917090924172827Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG31320.20296962972947752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45100.086.802371
ACGGGTA1950.079.780654
CGAAGGG11900.078.835962
GCGGGAT8500.077.647484
GTACGGG3950.077.577272
ATAGCGG2450.077.005891
AGTACGG2150.076.7820361
AGGGATG33250.075.4721155
TAGACGG2750.075.4657751
CGAGGGA9500.073.4443
TAAGGGA19050.073.2512363
AGGGCAT18350.072.987045
TAGGGCA11950.072.9830554
ATAGGGC10800.072.8972243
AAGGGAT38500.072.857544
AAGGGCA28400.072.706634
AAGGGAC19400.072.41574
AGAGGGC23750.072.054523
CGTAGGG4850.071.9296952
AGGGCAA20450.070.777355
Produced by FastQC (version 0.11.5)