FastQCFastQC Report
Mon 27 Feb 2023
SRR3127576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030917
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168820.8312501200196758No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG59720.29405436066565005No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG53060.26126129231278283No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC52050.256288169334345No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT25800.12703621073633242No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT23760.11699148709671543No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23130.11388944009036311No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT22650.11152597570457089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53500.084.671
ACGGGTA2750.083.7425164
GTACGGG3000.083.030422
CGTAGGG5150.080.307862
ATAGCGG4050.077.817421
TACGGGT1450.077.788463
CGAAGGG18200.077.469812
TAGGGCA15950.076.9063954
AGGGATG35650.076.726625
CGGGTAA1750.075.197365
AGGGCAT27400.074.957225
ATAACGG2600.074.176711
AAGGGCA39450.073.982194
GAATAGG14700.073.918251
AGGGATC25400.073.8281255
ATAGGGA29900.073.560823
CGAGGGA14700.073.533033
ATTGCGG3150.073.171611
AAGGGAT55200.073.1369254
TAGCGGG9000.073.108542