FastQCFastQC Report
Mon 27 Feb 2023
SRR3127575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412659
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231080.957781435337526No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG55660.23069982123457977No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG54390.2254359194565001No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC52110.21598576508325462No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29980.12426124039907836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG6350.088.810512
CGTTTTT76300.084.8418351
CGAAGGG22300.077.763962
AGGGATG41650.077.1787955
GCGGGAT15550.077.066674
GTACGGG4650.076.809962
AGGGCAT30950.076.073595
AAGGGCA45600.075.7494354
CGAGGGA17800.075.5097353
TAGCGGG11150.075.024312
ACGGGTA3950.074.9549564
TAGGGCA17400.074.544694
AGCGGGA20700.074.466413
GAGGGAT52050.074.3080754
ATGCGGG11000.073.91122
AAGGGAT69650.073.816444
AGGGATT59400.072.945985
ATAGCGG5050.072.664011
GCGTAGG3500.072.584161
AGGGATC32450.072.55715
Produced by FastQC (version 0.11.5)