FastQCFastQC Report
Mon 27 Feb 2023
SRR3127574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896396
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167380.8826215621631769No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG51410.27109316830451025No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG43820.23106988202885895No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC43100.22727320665093154No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22080.11643137825644011No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT21630.11405845614523549No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT21200.1117909972389733No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT20440.10778339545116104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55600.085.347121
CGTAGGG4850.083.525432
CGAAGGG16250.080.584852
ACGGGTA2650.079.988694
TAGGGCA13550.078.565364
TAGGGCG3100.077.494424
GCGGGAT11100.076.809974
AAGGGCA36500.076.657664
AGGGATG34900.076.484965
CGAGGGA13350.076.213943
AGGGCAT24000.076.1713265
AAGGGAT52150.075.872954
AGGGATT41900.075.147395
GTAGGGA21150.075.055323
AGCGGGA14000.074.694213
AGGGATC24300.073.877175
GTACGGG3450.073.728712
TAGCGGG7500.073.4829562
GAGGGAT40350.073.195774
AGGGAAC20750.073.1539155
Produced by FastQC (version 0.11.5)