FastQCFastQC Report
Mon 27 Feb 2023
SRR3127573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419775
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315261.3028484053269416No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG28910.11947391802956887No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC27860.11513467161203003TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG24220.10009195069789545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93150.087.287451
ATAGCGG5000.077.122931
CGTAGGG7250.074.542382
TAGGGCA17950.072.521654
AGTAAGG18100.070.9289861
TAAGGGA23650.070.342133
AGATAGG14950.070.146071
AGGGCAT26300.069.868655
AAGGGTA19050.069.567534
AAGGGCA47600.068.912964
TAAGAGG19150.068.7588961
AAGAGGG93200.067.970022
ATAGAGG17000.067.773021
AGGGATG39750.067.627025
ATAGGGA24500.067.518073
GAATAGG15850.067.053091
AAGGGAT45200.066.9575964
AGGGATC23250.066.704075
AAGGGAC25800.066.667444
AGTAGGG41950.065.98222
Produced by FastQC (version 0.11.5)