FastQCFastQC Report
Mon 27 Feb 2023
SRR3127572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927534
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416471.4225966291083212No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG29830.10189463213749182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125450.085.57241
ATAGCGG6100.073.262631
AGTAAGG21500.072.423291
TAGGGCA20450.072.165854
TAAGGGA30400.071.427233
CGTAGGG9200.071.010472
AGGGATG46650.069.616855
ATAGGGA27750.068.763583
ATGCGGG15450.068.750422
ATAGGGC22150.068.5368653
AAGAGGG112150.068.5194552
AAGGGAT55500.068.509534
GTACGGG7850.068.2543952
AGGGCAT31300.068.020825
TAGAGGG56850.067.3786852
GAGGGAT41800.067.3512954
ACGGGTA3350.067.34294
AGGGTAC13200.067.293925
AGAGGGT32050.067.163423
AAGGGCA56450.067.10684
Produced by FastQC (version 0.11.5)