FastQCFastQC Report
Mon 27 Feb 2023
SRR3127571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243638
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303111.3509755138752328No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG25610.11414497347611335No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC23580.10509716808148196TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93350.086.6112441
ATAGCGG4600.074.7715451
CGTAGGG5750.072.89042
ATAGGGC18500.072.8018653
TAGGGCA17600.072.778734
GAATAGG14700.072.757851
AGGGATG38350.071.81075
TAAGGGA23850.070.0951843
ATAGAGG14450.069.451731
TAGGGCG4550.069.344444
AGGGCAT24600.069.1560755
AGTAAGG14950.069.019891
AAGGGCA44800.068.9563144
ATAGGGA20200.068.773143
ATGCGGG11650.068.718112
GAGGGAT30200.068.143234
AGAGGGC40300.068.125823
AAGGGAT43100.067.851984
GTAGGGA16950.067.5125963
GTACGGG5450.067.3978962
Produced by FastQC (version 0.11.5)