FastQCFastQC Report
Mon 27 Feb 2023
SRR3127570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712163
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451241.6637643091510355No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30950.11411556016360375No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT30860.11378372170109245No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG30770.11345188323858117No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT28370.1046028575716135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148850.088.206581
ACGGGAT8750.077.876434
TAGGGCA19000.074.449014
ATAGGGC18550.074.228353
AGGGATG54150.073.680965
AAGGGAT55700.073.486754
TAGCGGG15000.072.05942
GGTAAGG14900.071.327431
AGGGCAT29550.071.2475
AGTAGGG49900.070.069092
AGGGATA35300.069.892515
GAGGGAT42250.069.629574
ATAGGGA28500.069.4197853
TAGAGGG47950.069.390292
TAGGGCG4550.069.200544
TAAGGGA26450.068.936963
GTAGGGA24450.068.6175843
AGAGGGC43750.067.886763
AAGGGCA45550.067.886534
TAGGGAT23600.067.504664
Produced by FastQC (version 0.11.5)