FastQCFastQC Report
Mon 27 Feb 2023
SRR3127569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236752
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586281.8113219672066319No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39030.12058384454539614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT196300.087.267331
AGGGATG63100.075.151585
TAGGGCA21350.072.862894
AAGGGAT67650.071.069644
TAAGGGA32750.071.0346453
ATAGGGC20900.069.709453
GAGGGAT51300.069.442954
AAGGGCA58000.069.2810444
CGTAGGG7750.069.130982
ATAGGGA36900.068.904443
AGAGGGC55500.068.6758653
TAGAGGG63850.068.084842
CGAAGGG31400.067.35232
AGGGCAT35100.067.082145
AGAGGGA88550.066.661853
GTAGGGA29600.066.209553
AGTAAGG21850.066.087441
ACGGGAT12250.065.6049044
GTACGGG6950.065.592862
AGTAGGG56850.065.473312
Produced by FastQC (version 0.11.5)