FastQCFastQC Report
Mon 27 Feb 2023
SRR3127568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527551
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434821.7203213703699747No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT29000.11473556814481686No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27380.10832620192431329No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG27090.10717884624286513No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT26280.10397416313261336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143650.088.521831
AGGGATG48550.074.146195
TAAGGGA25300.074.1161653
TAGGGCA16150.073.223694
ATAGGGA26900.073.035533
AAGGGAT51300.071.543554
GAGGGAT39100.071.4542854
GGTAAGG14600.070.3748861
CGTAGGG6000.069.885832
ATAGGGC16000.069.787683
GTAGGGA20400.069.0544053
AAGGGCA44900.069.044644
GTACGGG5050.068.105452
TAGGGAT23000.067.803254
AGTTAGG9400.067.689291
TAGCGGG14200.067.684972
AGTAGGG42350.067.639532
AGGGCAA28350.067.466895
TAGAGGG47300.067.334242
ACGGGTA2450.067.305784
Produced by FastQC (version 0.11.5)