FastQCFastQC Report
Mon 27 Feb 2023
SRR3127567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435367
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122970.8567146938727168No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG43120.3004109750328662Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG40500.28215780354432RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC39100.27240420045883734RNA PCR Primer, Index 41 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT15710.10944936033780908RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44500.083.51271
TAGGGCA10550.081.073814
AGGGATG24850.079.051765
GAGGGAT27800.078.2704544
ACGGGAT7650.078.019644
TAAGGGA17950.077.762243
GTAGGGA15250.077.6618043
AAGGGCA29850.077.1459964
AGGGATT29250.076.157755
AAGGGAT37600.075.993674
GAATAGG8050.074.7996441
AGAGGGC24600.074.126483
AGGGCAT19000.073.9569855
TACGAGG3350.073.020361
TAGCGGG7150.072.9614942
AGGGACC15150.072.898225
AGAGGGA46650.072.8389053
AGGGAAC17150.072.343825
AAGGGAC17400.072.114684
AGGGAAT18400.072.02665
Produced by FastQC (version 0.11.5)