FastQCFastQC Report
Mon 27 Feb 2023
SRR3127565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313410
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123020.93664583031955No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG38520.29328237184123773Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG36420.27729345748852224RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC33580.25567035426865947RNA PCR Primer, Index 41 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT14710.11199853815640204RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47350.085.564351
ATAGCGG2350.084.196841
TAGGGCA9000.081.64514
TAAGGGA17500.077.517993
AGGGCAT16750.076.595425
TAGGGCG2400.076.542294
TAACGGG3450.076.4569552
AAGGGCA25950.076.054474
GAGGGAT26200.076.047884
AGGGAAT20200.075.611345
AGGGATG24950.075.15495
AGGGATT27550.075.056115
ATAGGGC10500.074.467523
ATAGGGA17850.074.414743
GTAGGGA13950.073.946563
AGAGGGC22900.073.842613
GGTAAGG8950.073.691831
AAGGGAC16750.073.6774444
AAGGGAT35600.073.565284
AGAGGGA42900.073.564043
Produced by FastQC (version 0.11.5)