FastQCFastQC Report
Mon 27 Feb 2023
SRR3127564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975052
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81150.8322633049314293No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG29790.305522167022887No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC27030.27721598437826905No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG26680.2736264322313066No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT10140.10399445362913977No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10040.10296886730143623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT30900.088.5673751
GCGTAGG1250.082.760281
TAACGGG1600.082.247852
ATAGCGG1900.081.6713261
CGAAGGG8250.078.616122
TAAGGGA13350.078.507313
CGTAGGG2350.077.9979552
TAGCGGG4750.077.1769262
GGGCGAT3350.077.15826
ATAGGGA15500.075.804473
TAGGGCG1800.075.720244
GTACGGG1750.075.198032
CGAGGGA6550.074.6243
AGTACGG950.074.246661
AAGGGAT32600.074.102354
AGTAGGG16150.073.335542
ACGGGAT5650.073.201624
AGGGCGA4850.072.674785
AGGGATG21550.072.620835
AGGGATT22100.072.514755
Produced by FastQC (version 0.11.5)