FastQCFastQC Report
Mon 27 Feb 2023
SRR3127563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173521
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111780.9525181057688785No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG28280.24098418349565112No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG27840.23723478318666644No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC27540.234678373885086No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12110.10319372214046448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47400.087.236131
GTACGGG2050.082.5293962
TAGGGCG2000.082.242844
AGTACGG1050.080.643451
ACGGGTA1000.079.893054
CGTAGGG3200.079.3055952
CGGGTAT1400.077.2075655
ATAGCGG1900.076.752751
TAGGGAT19050.075.736184
TAAGGGA17200.075.412043
CGAGGGA8500.075.193453
AGGGATT27300.074.883595
CGAAGGG11400.074.616312
GCATAGG3700.073.741531
AGTAGGG19750.073.527782
ATAGGGA18700.073.383993
GAGGGAT25200.073.291244
AAGGGAT37850.073.132344
AGGGCGA5600.073.0115055
AGGGATG26550.072.396715
Produced by FastQC (version 0.11.5)