FastQCFastQC Report
Mon 27 Feb 2023
SRR3127562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925476
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79020.8538308935077734No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG26900.29066123810882183No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG24060.2599743267248421No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC23680.25586833153966176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2150.087.640462
CGTTTTT31450.086.593311
TAGGGCG1500.084.792134
CGAAGGG7550.081.734882
CGAGGGA6000.079.296343
ATAGCGG1550.079.034891
TAGCGGG3850.078.307312
ATAGGGA14750.077.606363
TAAGGGA13350.077.276223
GAGGGAT20350.076.389314
AGGGATG20750.076.0975345
TAGGGAT14600.075.8224954
AGGGATT21300.075.6775
GGGCGAT2850.074.2022556
AAGGGAC11050.074.177144
AGAGGGC13050.073.638123
AAGGGAT28750.073.568444
AGGGAAT14850.073.102975
GCATAGG3000.072.245991
AGTAGGG14000.072.006022
Produced by FastQC (version 0.11.5)