FastQCFastQC Report
Mon 27 Feb 2023
SRR3127561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093318
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104030.9515072467479726No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC26030.23808260725607738TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG25640.2345154840586179No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG24980.228478814032148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33850.087.131761
CGTAGGG3150.079.0743262
ATAGGGC7200.075.0612263
TAGGGCA8000.074.01694
TAGAGGG19950.073.970222
GAATAGG6800.073.327111
GGTAAGG6550.073.253181
ACGGGTA2000.072.842034
AGGGCAT10800.072.667975
GATTAGG3250.072.3692861
GTAGGGC5550.071.9741063
AAGGGTA7550.070.959124
AAGGGCG5450.069.845564
CTAGCGG950.069.322161
AGTAGGG18650.069.046542
CACCGAT1300.068.681659
TGTAGGG8850.068.504112
AGGGTAT7500.068.299195
AGTAAGG8850.068.03531
AGAGGGC15000.067.985893
Produced by FastQC (version 0.11.5)