FastQCFastQC Report
Mon 27 Feb 2023
SRR3127560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312277
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138571.055950839647422No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG26670.20323453051451792No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC25090.19119438959914714TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG23040.17557268777857113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG4350.087.509082
CGTTTTT44750.082.979461
TAGGGCA8900.077.621984
ATAGGGC10000.074.723043
GCGTAGG2200.072.73491
AAGGGTA10600.071.8234944
TAGGGTA6050.071.464414
TAGAGGG24550.071.211282
AGTAGGG22150.071.07692
ATAAGGG21500.070.384142
CTAGCGG1550.069.836551
GGTAAGG7200.069.2883151
AGTAAGG9900.068.931761
AGGGTAC5950.068.716285
TAGACGG1850.068.687651
ATAGAGG10600.068.3756561
AGGGCGA7450.068.127885
CGAAGGG10600.067.83332
GAATAGG10850.067.667711
AGAGGGC18850.067.563993
Produced by FastQC (version 0.11.5)