FastQCFastQC Report
Mon 27 Feb 2023
SRR3127559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006041
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.9806757378675421No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG22410.22275434102586278No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG22130.21997115425713265No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC21490.21360958450003528TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2750.085.641532
CGTTTTT30850.084.7857741
TAGAGGG18850.076.4640352
TAGGGCA6400.075.070164
ACGGGTA1700.074.81044
TAGGGCG1450.074.714864
ATAGCGG2400.074.62041
AGAGGGC13600.072.732333
TATAGGG8100.072.6895752
AGTAAGG6450.072.337031
CACCGAT1050.071.6116649
AGTAGGG17700.071.3195652
ATAGGGC7250.070.816693
GAATAGG6600.069.978931
AGGGCAT10800.069.622455
GTAGGGC5150.069.5109943
TAGGGTA4400.068.513234
AAGGGTA7700.068.513234
AGACGGG8050.068.460042
TAAGCGG2000.068.336571
Produced by FastQC (version 0.11.5)