FastQCFastQC Report
Mon 27 Feb 2023
SRR3127558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931821
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175700.9095045555462955No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC24470.12666805050778512Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG23200.12009394245118983Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22370.11579747813073779No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG21000.10870572377047356Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG6100.084.7454152
CGTTTTT59700.084.15021
TAGGGTA5400.074.8441854
TAGGGCG3850.073.239254
TAGGGCA12050.070.590284
GTACGGG5000.070.4927752
AGTAGGG31250.070.379992
GAATAGG12900.068.917631
TAGCGGG9950.068.485452
AGGGCGA10200.068.190865
TAGAGGG35600.067.4565662
GAGGGAT23250.066.500724
AAGGGCG9800.066.1768954
ATAGCGG4200.066.078611
AAGAGGG75400.065.568882
ATAGGGC13600.065.309493
TATAGGG14300.065.070262
ATGAGGG32150.064.7554242
AGGGTAA9200.064.364635
AGTAAGG10900.064.301041
Produced by FastQC (version 0.11.5)