FastQCFastQC Report
Mon 27 Feb 2023
SRR3127557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331863
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234611.0061054187145642No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27190.11660204737585354No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC24730.10605254253787638Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75900.084.522041
CGTAGGG7500.081.463432
TAGGGTA6900.073.5622944
GAATAGG15200.072.095761
ATAACGG2300.071.571081
TAGGGCA14300.071.318854
AGTAGGG39650.069.222982
GGTAAGG12400.068.272871
TAGCGGG11050.068.05162
ATAGGGC15000.067.6773153
AGGGCAT21600.067.015865
AATAGGG36950.066.1408162
ATAGAGG18900.065.944941
TAGAGGG49450.065.8639142
AGGGTAG10000.065.797395
AAGAGGG89800.065.47292
CGAAGGG22850.064.995232
TAAGGGA16600.064.834783
ACGGGTA2900.064.825014
GAGGGAT27350.064.783544
Produced by FastQC (version 0.11.5)