FastQCFastQC Report
Mon 27 Feb 2023
SRR3127555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141858
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346721.618781450497652No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG27870.13012067093149965No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT26980.12596540013390245No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC24500.11438666802374388No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT23460.10953107068722577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115100.086.503011
ACGGGTA1900.081.626594
TAGGGCA13250.076.968884
CGTAGGG6400.076.37392
ATAGGGA22050.075.2397843
ATAGGGC14800.074.308013
GAGGGAT37600.073.74554
GGTAAGG13100.072.521341
GAATAGG12050.071.424921
AGATAGG13050.071.357631
AGTAGGG37200.071.004362
AGGGATG36950.070.847125
GTAGGGA18800.070.497343
AGAGGGC39100.070.437243
TAGAGGG43800.070.284372
TAAGGGA24650.068.6383743
AAGGGAC25400.068.090334
AAGGGCA39950.067.995854
AAGGGAT50800.067.9053044
GCATAGG6400.067.60731
Produced by FastQC (version 0.11.5)