FastQCFastQC Report
Mon 27 Feb 2023
SRR3127554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585795
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462191.78741934298736No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG28570.11048826376414216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148550.088.056491
CGTAGGG6600.081.175372
TAGGGCA17200.075.139394
ATAGGGA24900.073.797353
ATAGGGC18150.073.5368653
GAGGGAT42950.072.436624
GTACGGG5650.072.365932
GAATAGG16600.070.554431
GGTAAGG14700.070.394451
TAAGGGA29850.070.218883
AGTAGGG43250.069.869592
ACGGGTA1750.069.823024
AGATAGG14350.069.8169251
AGGGATA36150.069.550535
AAGGGAT66700.069.402284
AGGGCAT32200.069.0335545
AGAGGGC45400.068.9416663
AGGGAAT29050.068.753975
ATAGCGG5550.068.647551
TAGAGGG52500.068.301252
Produced by FastQC (version 0.11.5)