FastQCFastQC Report
Mon 27 Feb 2023
SRR3127553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019919
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340581.686107215190312No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT25280.12515353338425947No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG24540.12149002014437212No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC21220.10505371750055324No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT21110.10450914120813755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109000.088.673251
TAGGGCA12650.075.248824
ACGGGTA1650.074.255434
CGTAGGG6750.074.0015642
GAATAGG11500.073.387221
ATAGAGG14300.072.206011
GAGGGAT32000.072.158074
TAGAGGG37350.071.410932
ATAGGGC12200.071.071713
AGTAGGG35050.070.8535162
GGTAAGG12000.070.722311
TAAGGGA21050.070.5175553
ATAGGGA18600.070.178753
AGGGCAT23100.069.575775
AGGGATG33800.069.378955
AAGGGAT46900.069.3290254
AAGGGTA13400.068.575444
AGAGGGA54850.067.871783
CGAAGGG20650.067.775892
AGGGATT32250.066.884715
Produced by FastQC (version 0.11.5)