FastQCFastQC Report
Mon 27 Feb 2023
SRR3127552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718186
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265040.9750620450550477No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG39790.14638439017786126No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC39510.1453542914281804No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG37070.136377716609533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.083.72931
CGTAGGG8200.080.812312
ATAGGGC19750.076.861293
ATAGCGG6650.075.682261
GAATAGG17250.075.2578661
TAGGGCA18050.073.423684
AGGGCAT28800.072.454075
CGAAGGG28350.071.449062
AGTAAGG19950.071.202621
AAGGGCA52300.069.461174
GGTAAGG16050.069.455281
ATAGGGA21350.069.340183
AGGGTAC12350.068.878535
AGAGGGC45450.068.350213
AGGGATG33350.067.360355
GTAAGGG46450.067.0810852
AGATAGG18200.066.677641
ACGGGAT5800.066.4431464
GTACGGG6750.066.144252
AATAGGG45150.066.097992
Produced by FastQC (version 0.11.5)