FastQCFastQC Report
Mon 27 Feb 2023
SRR3127551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290525
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355251.0796149550603626No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC36130.10980010788551979No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG35920.1091619118529718No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG35580.10812864208598932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113250.083.7744751
CGTAGGG11450.078.395122
GAATAGG21300.075.304141
TAGGGCA23550.074.634874
ATAGGGC22800.074.616493
AAGGGCA63250.071.701454
AGTAAGG20800.070.782371
AGGGCAT36150.070.721545
AAGGGTA26750.070.450124
AGTACGG5050.069.857641
AGAGGGC56050.069.760383
AGGGTAC17050.069.736045
CGAAGGG34450.068.7547152
GTAGGGA21650.067.943453
ATAGGGA27200.067.556793
AATAGGG55400.067.270492
ATAAGGG52300.067.124342
GTACGGG8900.067.0617452
AGTAGGG51300.066.8754352
ATGAGGG56100.066.43112
Produced by FastQC (version 0.11.5)