FastQCFastQC Report
Mon 27 Feb 2023
SRR3127550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473711
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257581.0412695743358864No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG35610.14395376016034211No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC34600.13987082565424983No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG34070.1377282956659044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79650.085.5207751
CGTAGGG7800.077.329732
AGGGTAC11150.073.7577445
AGGGCAT27650.073.593125
TAGGGCA19250.072.703754
ATAGGGC18550.072.398923
AAGGGCA47500.071.725874
TAGGGCG5050.070.917484
AGTAAGG16050.070.7834241
GAATAGG15400.070.403891
AGTACGG3550.070.378031
TAGGGTA9600.069.702484
ATAGGGA18800.069.681593
TAGACGG3550.069.050141
ATAGCGG5000.068.82441
AGAGGGC40350.068.786453
CGAAGGG24500.068.279982
AGGGCAC24300.068.073935
ATGAGGG40100.067.9226462
ATGCGGG11500.067.610982
Produced by FastQC (version 0.11.5)