FastQCFastQC Report
Mon 27 Feb 2023
SRR3127549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415517
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129410.9142242728275253No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG50460.3564775272921484No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG47370.3346480473212261No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC44570.3148672887715231No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT18100.1278684749105804No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT15640.11048966561334128No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15160.10709867843339217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46350.086.3532641
ATAGCGG2150.080.939811
AGGGATG26500.079.2725755
ACGGGAT5250.078.7743154
TACGGGT900.078.3295063
TAGCGGG5550.075.365692
CGTAGGG3200.074.9025962
AAGGGAT35100.074.711664
CGAAGGG11850.074.165152
AGGGCAT12750.073.3506855
AGGGAAT20550.073.181195
TAAGGGA15050.073.0728453
ATAGGGC8300.072.4783863
ATAACGG650.072.3578341
TAGGGCA8250.071.775774
AAGGGAC16650.071.693674
AGGGATT25850.071.630335
CGGGTAT1250.071.433985
AGTAGGG20550.071.354182
AGGGTAC4150.071.343395
Produced by FastQC (version 0.11.5)