FastQCFastQC Report
Mon 27 Feb 2023
SRR3127548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668234
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175461.0517709146318803No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG46790.2804762401437688No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG46390.2780784949833177No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC42440.254400761523863No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19570.11730968197507065No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT17020.10202405657719481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66500.087.0369641
AGGGATG32500.081.269235
ATAGCGG3300.078.427621
AAGGGAT44800.076.160864
CGTAGGG3800.075.4430852
AAGGGAC18950.074.4021154
TAAGGGA19200.073.6781163
TAGCGGG8200.072.788552
AGGGATT30950.072.735775
CGAGGGA9050.072.703053
GAGGGAT32400.072.236644
TAAGCGG3650.072.196381
GGGCGAT6400.071.964666
TCCGCTA3950.071.478331
GAAGGGA49600.071.254013
AGTAAGG8400.071.145061
CGGGAAT5750.071.109015
ATAGGGC10050.071.0805363
CGAAGGG14150.071.077242
ATAGGGA23200.070.293423
Produced by FastQC (version 0.11.5)