FastQCFastQC Report
Mon 27 Feb 2023
SRR3127547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305855
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126980.9723897369922389No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG43660.33434033640794725No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG41510.3178760275834607No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC39220.3003396242308679No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT15850.12137641621772707No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13660.10460579467092442No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT13630.10437606012918739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46950.086.384921
AATGCGG2000.080.0791241
ATAGCGG1250.079.1370241
AGGGATG25300.078.38755
AAGGGAC15250.077.515934
CGAAGGG11350.075.520112
GAGGGAT24700.075.125324
ATAGGGC6900.074.398553
ACGGGTA950.074.3626254
AGGGAAT19400.073.8844455
TAAGGGA14500.073.730123
ATAGGGA16100.073.7159963
AAGGGAT31550.073.4434054
CGTAGGG3150.073.260962
ACGGGAT5750.072.896934
AGGGATT22650.072.4122545
AGTAGGG19150.071.566752
AGAGGGA30800.071.561963
GGTAAGG8250.071.371771
GAAGGGA35550.070.611383
Produced by FastQC (version 0.11.5)