FastQCFastQC Report
Mon 27 Feb 2023
SRR3127546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561027
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225160.8791785482933214No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG54860.21421093959571685No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC53580.2092129446507202No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG51810.20230165476584197No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43430.16958040661031687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77750.080.4167251
CGTAGGG6500.077.37072
TAGACGG2250.075.273441
TAGGGCA15350.072.565314
TAGGGTA6900.072.201454
AGGGATG37900.071.924675
GAATAGG16750.071.622121
ATAGGGC16250.070.8614963
AGGGCAT27200.069.634635
AGTAGGG36600.069.088742
AAGGGTA19000.068.767044
TAGCGGG11950.068.436442
TAGGGCG4400.068.362264
GGTAAGG15650.068.2391051
AGGGTAC10900.068.1270755
ATAGGGA23700.067.2278063
TAGGGAT16950.067.101894
AATAGGG41350.067.062942
TAGAGGG45300.067.025552
CGAAGGG24350.066.399612
Produced by FastQC (version 0.11.5)