FastQCFastQC Report
Mon 27 Feb 2023
SRR3127545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070691
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309001.0062881611988963No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC54700.17813580070414117No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54700.17813580070414117No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG53170.17315320883801072No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG51110.16644462109668476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102400.083.1993941
CGTAGGG7700.076.293822
ACGGGTA2900.076.167544
AGGGCAC25950.069.543565
TAGGGTA9800.069.056814
TAGGGCA20100.068.741884
AGGGATG46850.068.6134955
AGTAGGG48300.068.208892
AGGGCAT32200.068.0131765
AAGAGGG107050.067.652852
GAATAGG20600.067.59681
TAGACGG2300.067.497511
CTACGAC12650.067.243625
ATAGGGC20650.067.1385653
AAGGGCA54050.067.126154
TAGAGGG55300.066.96862
TACGACC12800.066.822766
TAAGGGA28600.066.55173
ATGAGGG46150.066.396622
TAGCGGG14150.066.094722
Produced by FastQC (version 0.11.5)